Cytochrome P450’s (P450’s) catalyze the oxidative metabolism of most drugs and toxins. Although extensive studies have proven that some P450’s demonstrate both homotropic and heterotropic… Read More »NMR studies of ligand binding to P450(eryF) provides insight into the mechanism of cooperativity
The mechanism of CYP3A4-substrate interactions has been investigated using a battery of techniques including cysteine scanning mutagenesis, photoaffinity labeling, and structural modeling. In this study,… Read More »Probing the CYP3A4 active site by cysteine scanning mutagenesis and photoaffinity labeling
We cloned and characterized CYP709C1, a new plant cytochrome P450 belonging to the P450 family, that so far has no identified function except for clustering… Read More »Cloning, functional expression, and characterization of CYP709C1, the first sub-terminal hydroxylase of long chain fatty acid in plants. Induction by chemicals and methyl jasmonate
9-Hydroperoxy-18-hydroxyoctadec-10(trans)-enoic and 10-hydroperoxy-18-hydroxyoctadec-8(trans)-enoic acids deriving from type II (i.e. involving 1O2) photooxidation of 18-hydroxyoleic acid were detected after visible light-induced senescence experiments carried out with… Read More »Visible light-induced oxidation of unsaturated components of cutins: a significant process during the senescence of higher plants
The NRAMP 1 gene is a major candidate gene influencing the outcome of infections with intracellular pathogens in numerous species. NRAMP 1 is highly conserved… Read More »Structure of the bovine natural resistance associated macrophage protein (NRAMP 1) gene and identification of a novel polymorphism
Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) is a facultative intracellular bacterium and causal agent of Johne’s disease in cattle. Following phagocytosis, M. paratuberculosis resides and… Read More »Phagocytosis of M. paratuberculosis fails to activate expression of NADH dehydrogenase and nucleolin-related protein in bovine macrophages
Mycobacterium paratuberculosis (M. paratuberculosis) is a facultative intracellular bacterium with the ability to survive and proliferate inside the vesicles of macrophage cells. How M. paratuberculosis… Read More »Survival tactics of M. paratuberculosis in bovine macrophage cells
Complementary oligodeoxynucleotides (ODNs) that contain 2-aminoadenine and 2-thiothymine interact weakly with each other but form stable hybrids with unmodified complements. These selectively binding complementary (SBC)… Read More »Antisense oligonucleotides containing modified bases inhibit in vitro translation of Leishmania amazonensis mRNAs by invading the mini-exon hairpin
G/A motif triplex-forming oligonucleotides (TFOs) complementary to a 21 base pair homopurine/homopyrimidine run were conjugated at one or both ends to chlorambucil. These TFOs were… Read More »Factors influencing the extent and selectivity of alkylation within triplexes by reactive G/A motif oligonucleotides